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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

JoVE Journal
Biology

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JoVE Journal Biology

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

DOI:

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15:28 min

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October 01, 2010

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Bartosz Balana, Natalie Taylor, Paul A. Slesinger

¹Clayton Foundation Laboratories for Peptide Biology,Salk Institute for Biological Studies

Chapters

00:00Title
01:06Introduction
01:44Site Directed Mutagenesis and Preparation of Plasmid DNA for Transfection
03:09Plating of Cells, Transient Transfection, and Imaging of Transfected Cells
05:00Whole-cell Patch Clamping
12:57Representative Analysis
14:42Conclusion

Summary

Automatic Translation

We will demonstrate how to study the effect of a single point mutation on the function of an ion channel.

Tags

Mutagenesis Functional Analysis Ion Channels Heterologously Expressed Mammalian Cells Point Mutation GIRK Channels G Protein-gated Inwardly Rectifying Potassium Channels Excitability Of Neurons G Protein-coupled Receptors Muscarinic Receptor Alcohol Activation Amino Acid Mutation CDNA Sequencing Transfection HEK293T Cells Patch-clamp Electrophysiology Potassium Currents L257W Mutation

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