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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Article DOI: 10.3791/2210 • 10:58 min • December 3rd, 2010
December 3rd, 2010 •
*1, *1, 1
1Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill
* These authors contributed equally

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Summary
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We will demonstrate the setup and analysis of pre-microRNA 96-well arrays for QPCR using a robot as well as by hand with a Thermo Scientific Matrix multichannel pipette.

Tags

Profiling Pre-micro RNAs MicroRNAs Quantitative Real-time PCR QPCR Arrays Gene Expression Detection Limit Input Amounts Automated QPCR Setup Reproducibility High-throughput Experiments Profiling Of Genes Internal Plate Controls Experimental Variables Primer Pairs Small Non-coding RNAs Post-transcriptional Level Regulation Primary MiRNA Precursor MiRNA Mature MiRNA Levels
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