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抗原特异性在体内使用CFSE标记的靶细胞的杀伤含量
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
DOI:

09:49 min

November 09, 2010

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Chapters

  • 00:00Title
  • 01:13Habituating Animals
  • 01:29Eliciting Target-Specific Effector CTLs by Immunization (Day 0)
  • 03:03Program Operant Test Session
  • 03:16Harvesting Target Cells (Day 8)
  • 05:32Treatment and Labeling of Target Cells
  • 06:33Results: Operant Sensation Seeking Behavior
  • 07:39Adoptive Transfer and Subsequent Harvest of Labeled Cells
  • 08:06Conclusion
  • 08:20Flow Cytometry and Data Analysis
  • 08:36CFSE Profile Demonstrating Cell-Specific Killing in BALB/c Mice
  • 09:34Conclusion

Summary

Automatic Translation

许多感染引起强烈的CTL反应,但偶尔,响应细胞的数量不关联控制病原体<sup> 1</sup>。 CTL的质量的措施之一是他们杀了专门的能力<sup> 2</sup>。 CFSE标签的靶细胞,可用于研究这种CTL反应质量<em>在体内</em<sup> 3,4</sup>。

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