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Bioengineering

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JoVE Journal Bioengineering
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Article DOI: 10.3791/2306 • 13:49 min • January 11th, 2011
January 11th, 2011 •
1, 2, 1
1Department of Bioengineering, Rice University, 2Department of Molecular and Cellular Oncology, The Univeristy of Texas M. D. Anderson Cancer Center

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Summary
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In many biological and clinical situations it is advantageous to study cellular processes as they evolve in their native microenvironment. Here we describe the assembly and use of a low-cost fiber-optic microscope which can provide real time imaging in cell culture, animal studies, and clinical patient studies.

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High-resolution Fiber-optic Microendoscopy In Situ Cellular Imaging Longitudinal Study Morphology And Function Light Microscopy Intravital Techniques Confocal Microscopy Multiphoton Microscopy Second Harmonic Microscopy High-resolution Microendoscope Off-the-shelf Components Image Resolution Field-of-view Operating Wavelength End User In Vitro Cell Culture Excised Tissues Living Animal Tissues Human Tissues In Vivo Fluorescent Contrast Agents Proflavine Benzoporphyrin-derivative Monoacid Ring A (BPD-MA) Fluoroscein
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