Isolation and <em>In vitro</em> Activation of <em>Caenorhabditis elegans</em> Sperm

Gunasekaran Singaravelu; Indrani Chatterjee; Matthew R. Marcello; Andrew Singson

doi:10.3791/2336

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

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Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

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05:46 min

January 31, 2011

DOI: 10.3791/2336-v

Gunasekaran Singaravelu¹, Indrani Chatterjee¹, Matthew R. Marcello¹, Andrew Singson¹

¹Waksman Institute of Microbiology,Rutgers University

A protocol for isolating and activating spermatids from male C. elegans is described here. Cutting the posterior end of male releases spermatids. The spermatids can be activated by addition of protease.

The overall goal of this procedure is to visualize sperm morphology. This is accomplished by first isolating males for dissection one day in advance. The second step of the procedure is to dissect the males to release sperm.

The third step of the procedure is to visualize sperm morphology. The final step of the procedure is to prepare sperm for downstream applications. Ultimately, results can be obtained that show sperm morphology and the ability to activate through treatment with in vitro activators.

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