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Isolation and In vitro Activation of Caenorhabditis elegans Sperm
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Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Article DOI: 10.3791/2336 • 05:47 min • January 31st, 2011
January 31st, 2011 •
1, 1, 1, 1
1Waksman Institute of Microbiology, Rutgers University

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A protocol for isolating and activating spermatids from male C. elegans is described here. Cutting the posterior end of male releases spermatids. The spermatids can be activated by addition of protease.

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Caenorhabditis Elegans Isolation In Vitro Activation Sperm Males Hermaphrodites Gametogenesis Spermatheca Oocytes X Chromosome Non-disjunction Him Phenotype Tail Morphology Mating Structures Dissection Stereo Microscope Spermatids
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