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JoVE Journal
Immunology and Infection

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Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
 

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection

Article DOI: 10.3791/2349
November 19th, 2010

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Summary November 19th, 2010

Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.

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