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Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
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Chapters
- 00:00Title
- 00:20Introduction
- 01:20Preparations for Automated Sholl Analysis
- 02:14Tracing Neurons in NeuronJ
- 04:52Using Bonfire to Build Preliminary .swc Files from NeuronJ Data
- 06:27Using NeuronStudio to Finalize .swc Files
- 07:57Using ‘bonfire’ to Extract Morphological Data and ‘bonfire_results’ to View the Data
- 09:06Using ‘bonfire_export’ to Export Data into Excel
- 11:21Conclusion
We have developed a computer program to analyze neuronal morphology. In combination with two existing open source analysis tools, our program performs Sholl analysis and determines the number of neurites, branch points, and neurite tips. The analyses are performed so that local changes in neurite morphology can be observed.
Tags
Automated Sholl AnalysisDigitized Neuronal MorphologyMultiple ScalesNeuronsFunctionCommunicationInputsAction PotentialsNeuritesInformation ProcessingDendrite MorphologiesSignalingSpecialized FunctionsNeuronal NetworkAlterations In MorphologyFragmentationBranching PatternsDisease StatesAlzheimer’s DiseaseSchizophreniaMental RetardationFactors Shaping Dendrite MorphologiesNervous System Function And DysfunctionSholl AnalysisCounting Neurites And Branch TipsAxonsTime-consuming AnalysisVariabilityExperimenter BiasInconsistencyBonfire Program
