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生产的灭鼠疟疾寄生虫的遗传交叉议定书“
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Immunology and Infection
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JoVE Journal Immunology and Infection
Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
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生产的灭鼠疟疾寄生虫的遗传交叉议定书“

DOI:
10.3791/2365-v

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13:39 min

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January 03, 2011

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, ,
1National Institute of Allergy and Infectious Diseases,National Institutes of Health, 2School of Life Science,Xiamen University

Chapters

  • 00:05Title
  • 01:16Introduction
  • 01:37Infection of Laboratory Mice with Blood-Stage Malaria Parasites
  • 04:06Mixed Clone Infection of Mice
  • 05:14Feeding Mosquitoes
  • 06:45Dissecting Mosquito Midget and Counting Oocysts
  • 08:58Collecting Sporozoites from Mosquitoes by Centrifugation
  • 10:56Cloning Progeny from a Genetic Cross Using Limiting Dilution
  • 12:57Production of Recombinant Malaria Parasites by Genetic Cross
  • 13:11Conclusion

Summary

Automatic Translation

Automatic Translation

啮齿类动物疟疾寄生虫的基因杂交进行喂养蚊子的两个基因的不同的寄生虫。克隆小鼠血液，让蚊虫叮咬感染小鼠后，重组的后代。本视频演示了如何产生遗传杂交<em>约氏疟原虫</em>，并适用于其他啮齿类动物疟疾寄生虫。

Tags

Genetic CrossRodent Malaria ParasitesAntimalarial DrugsLinkage MappingGenesLociPlasmodium YoeliiLaboratory AdaptationBiologic CharacteristicsGenetic MarkersMicrosatellite MarkersAFLP MarkersPlasmodium FalciparumTechniquesDrs. David WallikerRichard CarterUniversity Of EdinburghMice Mus MusculusGametocytesPhenotypesMosquitoes

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