Injection of Fluorescent Neural Progenitor Cells into Human Cerebral Organoids for Transplantation Modeling

Begin with fluorescently labeled induced pluripotent stem cell-derived neural progenitor cells or NPCs.

Resuspend the cells in an extracellular matrix hydrogel to create a uniform cell-matrix mixture.

Incubate the mixture on ice.

Next, transfer a human cerebral organoid into a dish.

Remove excess media and place the dish under a microscope for the injection procedure.

Dispense the cell-matrix mixture onto a pre-chilled, sterile glass slide, and aspirate it using a pre-chilled syringe.

Slowly inject the mixture onto the organoid surface to minimize tissue disruption.

Let the organoid rest briefly, then add media, transfer the organoid to a multi-well plate, and incubate.

The hydrogel solidifies, anchoring the NPCs at the injection site. Track the NPCs using fluorescence microscopy.

Over time, the NPCs proliferate and migrate to distant regions of the organoid, where they differentiate into immature and mature neurons.

This enables reliable cell transplantation with minimal organoid disruption, suitable for neural cell therapy.

Begin by removing the single cell suspension of induced pluripotent stem cells or iPSC-derived neural progenitor cells, or NPCs, from the ice and centrifuging them at 30