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JoVE Journal Neuroscience
Märkning F-aktin Barbed Slutar med Rhodamine-aktin i Permeabilized Neuronal Tillväxt Kottar
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Märkning F-aktin Barbed Slutar med Rhodamine-aktin i Permeabilized Neuronal Tillväxt Kottar

Article DOI: 10.3791/2409-v • 09:15 min • March 17th, 2011
March 17th, 2011 •
1, 1
1Department of Neuroscience, University of Minnesota

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En metod att visualisera och kvantifiera F-aktin taggtråd slutar i neuronala tillväxt kottar beskrivs. Efter odling nervceller på glas täckglas, celler permeabilized med en saponin-innehållande lösning. Sedan innehåller en kort inkubation med saponin buffert innehållande Rhodamine-aktin fluorescerande aktin på fri aktin hullingar slutar.

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Neurovetenskap Actin tillväxt kottar hullingförsedda slutar polymerisation vägledning cues
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