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Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
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JoVE Journal Neuroscience
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

DOI:
10.3791/2457-v

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11:54 min

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January 11, 2011

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, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Chapters

  • 00:01Title
  • 00:29Introduction
  • 00:57Animal Dissection
  • 02:40E14 Brain Dissection
  • 04:42Tissue Dissociation
  • 08:00Passaging Neurospheres
  • 10:10Representative Results
  • 11:31Conclusion

Summary

Automatic Translation

Automatic Translation

This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

Tags

Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres

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