JoVE Encyclopedia of Experiments
Microbiology
0 views • 3:42 min • September 30th, 2026
Begin with human epithelial-like cells.
The first well contains non-transfected cells, and the second well contains microRNA mimic-transfected cells.
In transfected cells, a microRNA mimic strand binds with a protein complex to form an RNA-induced silencing complex.
Remove the media and add the viral suspension.
Incubate to allow viral attachment.
Discard the media and add fresh media, then incubate.
In non-transfected cells, the virus undergoes receptor-mediated endocytosis and enters the cell.
It releases the viral genome which replicates and produces many viral particles.
In transfected cells, the RNA-induced silencing complex recognizes the viral genome, triggering its degradation and reducing virus production.
New viruses are released. Transfer the virus-containing media.
Centrifuge to pellet the debris and collect the virus-containing supernatants.
Add the supernatant to non-transfected cells. Incubate for viral infection.
Wells treated with non-transfected cell supernatant show higher cell death, compared to wells treated with microRNA mimic-transfected cell supernatant, confirming microRNA-mediated inhibition of viral replication.
In this step, plate the permissive cells in a 96-well ti
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