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JoVE Journal Biology
RNAi Interference by dsRNA Injection into Drosophila Embryos
Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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RNAi Interference by dsRNA Injection into Drosophila Embryos

Article DOI: 10.3791/2477-v • 08:31 min • April 11th, 2011
April 11th, 2011 •
1, 1, 1, 1
1Department of Biological Sciences, Oakland University

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RNA interference has been proven very effective to analyze gene function in Drosophila tracheal development. A detailed protocol used by Jiang lab to inject dsRNA into fly embryos to knockdown gene expression is illustrated. This technique has the potential for screening genes required for tissue and organ development in Drosophila.

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RNAi Interference DsRNA Injection Drosophila Embryos Genetic Screening Genetic Manipulation Reverse Genetic Approach RNAi Gene Functions Organ Development Tubular Networks Tracheal Formation Morphogenesis Gene Expression Knockdown Dysfusion Gene
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