Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Whole Cell Recording from an Organotypic Slice…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
Ex vivo Culturing of Whole, Developing Drosophila Brains
Journal (Neuroscience)
Ex vivo Culturing of Whole, Developing Drosophila Brains
Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
Journal (Neuroscience)
Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
 

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Article DOI: 10.3791/2600 • 10:29 min • June 3rd, 2011
June 3rd, 2011 •
1, 1, 1, 1
1Department of Anatomy and Neurobiology, University of Tennessee Health Science Center

Chapters

Summary
Automatic Translation

This is a protocol to prepare and maintain a neocortical slice preparation in organotypic culture for the purpose of making electrical recordings from pyramidal neurons.

Tags

Whole Cell Recording Organotypic Slice Preparation Neocortex Voltage-gated Potassium Channels Pyramidal Neurons Genetic Approach Organotypic Culture Preparation Laminar Pattern Of Cortex Acute Neocortical Slices Visually-identified Pyramidal Neurons Infrared Illumination Differential Interference Contrast Microscopy Whole Cell Patch Clamp Current-clamp Voltage-clamp Biolistic Transfection Wild Type Potassium Channel DNA Mutant Potassium Channel DNA Function Study Epifluorescence Microscopy Green Fluorescent Protein (GFP) Adjacent Untransfected Neurons
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter