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Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
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Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Article DOI: 10.3791/2600-v • 10:29 min • June 3rd, 2011
June 3rd, 2011 •
1, 1, 1, 1
1Department of Anatomy and Neurobiology, University of Tennessee Health Science Center

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This is a protocol to prepare and maintain a neocortical slice preparation in organotypic culture for the purpose of making electrical recordings from pyramidal neurons.

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Whole Cell Recording Organotypic Slice Preparation Neocortex Voltage-gated Potassium Channels Pyramidal Neurons Genetic Approach Organotypic Culture Preparation Laminar Pattern Of Cortex Acute Neocortical Slices Visually-identified Pyramidal Neurons Infrared Illumination Differential Interference Contrast Microscopy Whole Cell Patch Clamp Current-clamp Voltage-clamp Biolistic Transfection Wild Type Potassium Channel DNA Mutant Potassium Channel DNA Function Study Epifluorescence Microscopy Green Fluorescent Protein (GFP) Adjacent Untransfected Neurons
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