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Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)
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Bioengineering
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JoVE Journal Bioengineering
Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

DOI:
10.3791/2605-v

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07:33 min

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June 01, 2011

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, , , , , , , , ,
1Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg,University Heart Center Hamburg, 2Department of Experimental and Clinical Pharmacology and Toxicology,University Heart Center Hamburg, 3CT Surgery,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:26EHT Generation and Culture Conditions
  • 03:00EHT Implantation
  • 04:57Bioluminescence Imaging of EHT following Implantation
  • 06:08Correlation of BLI Results and ELISPOT
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

Tags

Bioluminescence ImagingAssessmentImmune ResponsesImplantationScaffolding StrategiesNative Scaffold MaterialsBioartificial Scaffold MaterialsCardiac MyocytesHydrogelsFibrinCollagenMyocyte MonolayersRestoration Of Cardiac FunctionMyocardial InfarctionExperimental ModelsPredictive ToxicologySubstance ScreeningDisease ModellingCell Survival MonitoringIn-vivo Bioluminescence Imaging (BLI) TechniquesFibrin-based EHT GenerationTransgenic Rat StrainFirefly Luciferase ExpressionROSA/luciferase-LEW Tg StrainOmentum ImplantationImmune Responses AssessmentBLI Results Comparison With ELISPOT Technique

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