Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Bimolecular Fluorescence Complementation
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Bimolecular Fluorescence Complementation
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems
Journal (Biology)
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems
BioMEMS and Cellular Biology: Perspectives and Applications
Journal (Biology)
BioMEMS and Cellular Biology: Perspectives and Applications
 

Bimolecular Fluorescence Complementation

Article DOI: 10.3791/2643 • 08:54 min • April 15th, 2011
April 15th, 2011 •
1, 1
1Department of Pharmacology, University of Illinois at Chicago

Chapters

Summary
Automatic Translation

The subcellular localization of proteins is important in determining the spatio-temporal regulation of cell signaling. Here, we describe bimolecular fluorescence complementation (BiFC) as a straightforward method for monitoring the spatial interactions of proteins in the cell.

Tags

Bimolecular Fluorescence Complementation Subcellular Distribution Signaling Complexes Immunoprecipitation Spatial Localization BiFC Protein Complexes Fluororescent Protein Amino-terminal Carboxy-terminal Protein Fusion Interaction Reconstitution Fluorophore Limitation Transient Interactions Weak Interactions Kinetic Analysis Complex Dynamics Flourophore Maturation Real Time Interactions Protein Fragment Complementation Assay Reporter Proteins Green Fluorescent Protein Variants Dihydrofolate Reductase B-lactamase Luciferase Protein:protein Interactions Fluorescence Co-localization Förster Resonance Energy Transfer (FRET)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter