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Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
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JoVE Journal Biology
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

DOI:
10.3791/51327-v

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11:10 min

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March 09, 2014

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1Department Biologie I, Botanik,Ludwig-Maximilians-Universität, München

Chapters

  • 00:05Title
  • 01:18Transient Transformation of Tobacco Leaves
  • 02:59Isolation of Protoplasts
  • 04:52Laser Scanning Microscopy
  • 07:18Results: BiFC Enables Visualization of Protein-protein Interactions in Tobacco Leaf Cells
  • 10:45Conclusion

Summary

Automatic Translation

Automatic Translation

Formation of protein complexes in vivo can be visualized by bimolecular fluorescence complementation. Interaction partners are fused to complementary parts of fluorescent tags and transiently expressed in tobacco leaves, resulting in a reconstituted fluorescent signal upon close proximity of the two proteins.

Tags

Bimolecular Fluorescence Complementation (BiFC)Protein-protein InteractionsTobacco Protoplasts And LeavesIn Vivo VisualizationSubcellular LocalizationMarker GenesConfocal MicroscopyAgrobacterium-mediated TransformationLimitations Of BiFC

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