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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

DOI:
10.3791/2649-v

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09:45 min

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June 03, 2011

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, , , , , , , ,
1Department of Zoology,University of Oklahoma – Norman, 2Department of Biology,Brandeis University

Chapters

  • 00:05Title
  • 01:45Mapping Transgenic Flippase CNS Expression Patterns in Larva
  • 03:37Mapping Transgenic Flippase CNS Expression Patterns in Adults
  • 05:57Determining the Cell Types in which a Transgenic Flippase Expresses
  • 06:57Utilizing FLP Expression in Photoreceptors from ET-FLP-x2 Lines
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

Tags

Gal4/UAS Binary MethodGene ManipulationNeural Circuitry ManipulationFINGR MethodGal4 ExpressionGal80 Converting ToolsEnhancer-trap FLPUAS-effectorUAS-reporterMorphological AnalysisTubP>Gal80> (‘flip Out’)TubP>stop>Gal80 (‘flip In’)

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