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Neuroscience

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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
 

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Article doi: 10.3791/2649
June 3rd, 2011

Summary June 3rd, 2011

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

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