Enhancer-trap Flp
Enhancer-trap Flp - Related Videos
09:32
Polycrystalline Silicon Thin-film Solar cells with Plasmonic-enhanced Light-trapping
0 Views •
Cited by 9 •
2012
Polycrystalline silicon thin-film solar cells on glass are fabricated by deposition of boron and phosphorous doped silicon layers followed by crystallisation, defect passivation and metallisation. Plasmonic light-trapping is introduced by forming Ag nanoparticles on the silicon cell surface capped with a diffused reflector resulting in ~45% photocurrent enhancement.
09:45
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
0 Views •
Cited by 7 •
2011
We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.
08:18
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
0 Views •
Cited by 8 •
2011
The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.
12:13
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
0 Views •
Cited by 37 •
2012
A quick method to modify the genome of V. cholerae is described. These modifications include the deletion of single genes, gene clusters and genomic islands as well as the integration of short sequences (e.g. promoter elements or affinity-tag sequences). The method is based on the natural transformation and FLP-recombination.
13:34
Gene Trapping Using Gal4 in Zebrafish
0 Views •
Cited by 19 •
2013
This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.
FAQs
Targeting specificity comes from the regulatory activity of the enhancer captured by the trap. Flp is expressed in cells where that enhancer is active, so the resulting genetic label or manipulation follows the enhancer’s activity pattern rather than a broadly distributed neuronal marker. This makes the system useful for distinguishing neural populations with different regulatory programs.
FRT-site orientation determines what happens after Flp recognizes the target DNA. In one arrangement, Flp excises the intervening sequence; in the opposite orientation, it inverts that sequence. This distinction allows the same recombinase system to support different genetic outcomes, including permanent removal or rearrangement of a DNA segment linked to a reporter or effector.
Enhancer-trap Flp can be combined with other genetic targeting elements so that a reporter or effector becomes associated with cells meeting more than one targeting condition. In this intersectional strategy, enhancer-driven Flp provides one layer of specificity, while an FRT-dependent construct supplies the corresponding readout or manipulation. The combination helps define neuronal populations more precisely than either element alone.
A typical workflow pairs an enhancer-trap Flp driver with an FRT-dependent reporter or effector. Researchers then examine which cells acquire the genetic label or manipulation after Flp acts on the FRT sites. The resulting pattern identifies the enhancer-defined population and provides a basis for mapping, labeling, or controlling its neural-circuit contribution.
Researchers can use Enhancer-trap Flp when they need to distinguish or manipulate neuronal populations defined by regulatory DNA activity. Supported applications include neural-circuit mapping, developmental studies, and investigations of how genetically distinct neurons contribute to behavior and brain function. Its value is greatest when a broad neural population must be resolved into more specific genetically defined groups.
The system can produce identifiable cell populations through FRT-dependent reporters or alter selected cells through FRT-dependent effectors. These outcomes let researchers connect enhancer-defined neuronal groups with circuit organization, developmental patterns, behavior, or brain function. Because Flp-dependent DNA rearrangement can generate labeling or manipulation, the same targeting logic can support both descriptive and functional experiments.