02:00Preparation of PBMC and Bulk Alloanergizing Cultures
03:57Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction
06:48Measuring the Specificity of Alloanergizing
09:05Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation
09:43Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR
This paper describes a simple technique to induce alloantigen-specific anergy in human peripheral blood mononuclear cells. The technique can be applied clinically to generate non-alloreactive donor cells. Infusion of these cells could improve immune reconstitution and reduce toxicity after allogeneic hematopoietic stem cell transplantation.