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在 C 在体内激光干切断线虫
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In vivo Laser Axotomy in C. elegans
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在 C 在体内激光干切断线虫

DOI:
10.3791/2707-v

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09:18 min

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May 19, 2011

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, ,
1Department of Genetics, Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine

Chapters

  • 00:05Title
  • 01:02Setting Up the Laser Ablation System
  • 02:17Focusing the Laser: Z-plane
  • 04:12Focusing the Laser: X-Y Place
  • 05:00Immobilizing Worms for Laser Ablation
  • 06:05Performing Laser Axotomies
  • 07:57Scoring Neurons for Regeneration
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

减少神经元的协议<em> C。线虫</em>与MicroPoint脉冲激光。我们描述了建立该系统，固定蠕虫，并切断标记的神经元。优势包括成本相对较低的系统和能力，切断神经元突起或消融细胞<em>在体内</em>。

Tags

In Vivo Laser AxotomyC. ElegansNeuronsRegenerationModel OrganismGenesSignaling PathwaysLaser-mediated CuttingAxotomyFluorescently-labeled Neuronal ProcessFemtosecond LaserPulsed LaserMicroPoint Pulsed LaserTargeted Cell Ablation

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