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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
 

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Article doi: 10.3791/2721
May 26th, 2011

Summary May 26th, 2011

An efficient procedure to assess the oligomerization propensity of single-pass transmembrane domains (TMDs) is described. Chimeric proteins consisting of the TMD fused to ToxR are expressed in an E. coli reporter strain. TMD-induced oligomerization causes dimerization of ToxR, activation of transcription and production of the reporter protein, -galactosidase.

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