A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:41Introduction
- 00:54Starvation Treatment for Adult Flies
- 01:26Insulin Injection Preparation
- 02:48Insulin Injection Procedure
- 03:52Hemolymph Collection and Glucose Determination
- 04:55Representative Insulin Tolerance Response Determined by Spectrophotometry
- 05:13Conclusion
Conserved insulin signaling pathways found in the fruit fly Drosophila melanogaster make this organism a potential tool for modeling metabolic disorders including type II diabetes. To this end, it is critical to establish physiological assays to effectively measure systemic insulin action in peripheral glucose disposal in the adult fly.
