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Journal / Biology / Live Cell Imaging mit Calcium siRNA vermittelte…
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Biology

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JoVE Journal Biology
Live Cell Imaging mit Calcium siRNA vermittelte Gene Silencing Combined Identifiziert Ca 2 + Leck-Kanäle in der ER-Membran und deren Regulationsmechanismen
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Live Cell Imaging mit Calcium siRNA vermittelte Gene Silencing Combined Identifiziert Ca 2 + Leck-Kanäle in der ER-Membran und deren Regulationsmechanismen

Article DOI: 10.3791/2730-v • 13:41 min • July 7th, 2011
July 7th, 2011 •
*1, *1, 2, 1
1Medical Biochemistry and Molecular Biology, Saarland University, 2Experimental and Clinical Pharmacology and Toxicology, Saarland University
* These authors contributed equally

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Das endoplasmatische Retikulum spielt eine wichtige Rolle in Protein Biogenese und in Calcium-Homöostase. Wir haben ein experimentelles System, das uns auf die Rolle der Ca2 +-Kanäle Leck-Adresse und ihrer vermeintlichen Regulationsmechanismen charakterisieren lässt etabliert. Dieses System beinhaltet siRNA-vermittelten Gen-Silencing-und Live-Cell-Ca2 +-Imaging.

Tags

Cell Biology Ausgabe 53 Cellular Calcium-Homöostase Calmodulin Ergänzung endoplasmatisches Retikulum ER Kalzium Leckage Gen-Silencing IQ-Motiv Mutanten-Analyse Sec61-Komplex
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