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Immunology and Infection
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Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

DOI:
10.3791/2736-v

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08:52 min

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May 27, 2011

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, , ,
1Center for Cell and Gene Therapy,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:49DC Nucleofection
  • 04:01T Cell Stimulation
  • 06:18G-Rex Expansion of Multivirus-specific T Cells Generates High Numbers of Polyfunctional, Antigen-specific Effector T Cells
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

A rapid, simple and cost-effective protocol for the generation of donor-derived multivirus-specific CTLs (rCTL) for infusion to allogeneic hematopoietic stem cell transplant (HSCT) recipients at risk of developing CMV, Adv or EBV infections. This manufacturing process is GMP-compliant and should ensure the broader implementation of T-cell immunotherapy beyond specialized centers.

Tags

Multivirus-specific T CellsViral InfectionsAllogeneic Hematopoietic Stem Cell TransplantEpstein Barr VirusCytomegalovirusAdenovirusAntigen Presenting CellsCytotoxic T LymphocytesGMP-compliant CTL Production ProtocolDendritic CellsDNA PlasmidsIL-4IL-7

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