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Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
This content is Free Access.
1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering,Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism
Chapters
- 00:05Title
- 02:14Brain Slice Preparation
- 03:22Flame-Sealed Glass Micropipette Preparation and Vibrodissociation
- 05:14Electrophysiological Recording
- 06:39Representative Results for Presynaptic Terminal Imaging and Synaptic Transmission Recording
- 07:51Conclusion
This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording.
Tags
VibrodissociationNeuronsRodent Brain SlicesSynaptic TransmissionPresynaptic TerminalsMechanical DissociationIsolated NeuronExtracellular EnvironmentPostsynaptic Intracellular EnvironmentMicropipetteVibration-based TechniqueP1-P21 RodentLiberation Of Isolated NeuronsPrimary Neuronal CulturesEnzymatically Isolated NeuronsElectrophysiological StudiesImaging StudiesPharmacological ExperimentsRapid Drug ApplicationTotal Cell SuperfusionWhole-cell Recordings
