Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Sang Beom Jun; Verginia Cuzon Carlson; Stephen Ikeda; David Lovinger

doi:10.3791/2752

Journal

/

Neuroscience

/

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

15,477 Views

•

08:38 min

•

May 25, 2011

DOI:

10.3791/2752-v

DOI:

10.3791/2752-v

•

08:38 min

May 25, 2011

•

6 Views

Sang Beom Jun^1,2, Verginia Cuzon Carlson¹, Stephen Ikeda³, David Lovinger¹

¹Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, ²Department of Electronics Engineering,Ewha Womans University, ³Section on Transmitter Signaling/Laboratory of Molecular Physiology,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

Summary

This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording.