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从石蜡包埋材料的遗传和表观遗传分析的DNA提取

JoVE Journal
Biology
Author Produced

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JoVE Journal Biology

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

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从石蜡包埋材料的遗传和表观遗传分析的DNA提取

DOI:

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13:32 min

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March 26, 2011

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Larissa A. Pikor*^1,2, Katey S. S. Enfield*^1,2, Heryet Cameron, Wan L. Lam^2,4

¹Department of Integrative Oncology,BC Cancer Research Centre, ²Interdisciplinary Oncology Program,University of British Columbia – UBC, ³Photography/Video Production, Multi-Media Services,BC Cancer Agency, ⁴Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Chapters

00:05Title
00:38Paraffin Removal
01:40Ethanol Rehydration
02:58Tissue Digestion
04:09DNA Clean Up
07:12DNA Precipitation
10:40DNA Quantification

Summary

Automatic Translation

本视频演示了从福尔马林固定石蜡包埋的材料DNA提取的协议。这是一个多天的过程中，水化组织切片用二甲苯deparaffinized，乙醇和蛋白酶K处理，净化和隔离随后的特定基因或全基因组分析的DNA。

Tags

DNA Extraction Paraffin Embedded Material Genetic Analysis Epigenetic Analysis Chromosomal Rearrangements Copy Number Gains And Losses DNA Methylation High-throughput Profiling Technologies Microarrays Sample Quality Specimen Collection Formalin-fixed And Paraffin Embedded (FFPE)Hospital Archives Extraction Method Xylene Treatment Ethanol Washes Proteinase K Lysis Buffer

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