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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
 

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Article doi: 10.3791/2783
May 16th, 2011 •
1, 1, 1, 1, 1
1Universite de Montreal, Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire

Summary
Automatic Translation
May 16th, 2011

This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.

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