Journal
/
Immunology and Infection
/
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

DOI:
10.3791/2785-v

•

07:34 min

•

June 02, 2011

•

, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Chapters

  • 00:05Title
  • 01:30Dissection of Mouse Femurs and Tibiae and Culture of Bone Marrow Cells
  • 03:59Collection and Labeling of DCs
  • 05:10Results: Qdot Nanocrystal Staining of DCs
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

Dendritic cells uptake antigens and migrate towards immune organs to present processed antigens to T cells. Qdot nanocrystal labeling provides a long-lasting and stable fluorescent signal. This allows tracking of dendritic cells to different organs by fluorescent microscopy.

Tags

GenerationLabelingMurineBone Marrow-derived Dendritic CellsQdot NanocrystalsTracking StudiesAntigen Presenting CellsImmunological OrgansMajor Histocompatibility MoleculesMigratory CapabilitiesFluorescent DyesQdot Fluorescent NanocrystalsStable FluorescenceCell CultureDifferentiationIn Vitro IncubationFluorescent MicroscopyExperimental AnimalsMature Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2785?article_type=v

Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
check_url/2785?article_type=v

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
check_url/2785?article_type=v

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
check_url/2785?article_type=v

Bone Marrow-derived Macrophage Production
check_url/2785?article_type=v

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
check_url/2785?article_type=v

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
check_url/2785?article_type=v

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
check_url/2785?article_type=v

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
check_url/2785?article_type=v

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
check_url/2785?article_type=v

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Read Article