A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:10Harvesting of Tissues
- 03:03Preparation of Fetal Liver Cells
- 05:01Antibody Staining for Flow Cytometry
- 07:14Identification and Analysis of Erythroid Progenitors
- 13:52Conclusion
A flow-cytometric method for identification and molecular analysis of differentiation-stage-specific murine erythroid progenitors and precursors, directly in freshly –harvested mouse bone marrow, spleen or fetal liver. The assay relies on cell-surface markers CD71, Ter119, and cell size.
Tags
IdentificationAnalysisMouse Erythroid ProgenitorsCD71/TER119 Flow-cytometric AssayErythropoiesisRed CellsHematopoietic ProgenitorsErythropoietinErythropoietic Stress ResponseCirculating Red CellsErythropoietic Stress ResponsePhysiological ConditionsPathological ConditionsMouse ModelDefinitive ErythropoiesisFetal LiverNeonatal SpleenAdult SpleenBone Marrow
