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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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06:09 min

July 31, 2011

DOI:

06:09 min
July 31, 2011

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Summary

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Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) couples affinity enrichment of peptides with stable isotope dilution mass spectrometry (MRM-MS) to provide quantitative measurement of peptides as surrogates for their respective proteins. Here we describe the protocol using magnetic particles in a partially automated format.

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