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Neuroscience

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JoVE Journal Neuroscience
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
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Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Article DOI: 10.3791/2821
June 28th, 2011 •
1, 1, 1
1Department of Pathology and Immunology, Washington University School of Medicine

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June 28th, 2011

This protocol describes a simple and inexpensive way to quantify the activity of cis-regulatory elements (i.e., enhancer/promoters) in living mouse retinas via explant electroporation. DNA preparation, retinal dissection, electroporation, retinal explant culture, and post-fixation analysis and quantification are described.

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