Journal
/
Neuroscience
/
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

DOI:
10.3791/2821-v

•

07:38 min

•

June 28, 2011

•

, ,
1Department of Pathology and Immunology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:30Eye Collection
  • 02:43Retinal Dissection
  • 03:58Preparation for Electroporation
  • 04:34Electroporation
  • 05:27Culturing of Electroporated Retinas
  • 06:23Visualization of cis-regulated Element (CRE) Expression in Electroporation Retinal Cells
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a simple and inexpensive way to quantify the activity of cis-regulatory elements (i.e., enhancer/promoters) in living mouse retinas via explant electroporation. DNA preparation, retinal dissection, electroporation, retinal explant culture, and post-fixation analysis and quantification are described.

Tags

Cis-regulatory ElementsMouse RetinaExplant ElectroporationTranscription FactorsGene NetworksEnhancersPromotersTranscriptional ActivatorsTranscriptional RepressorsSpatiotemporal PatternGene ExpressionGenomic DNATransgenesisEnhancer FunctionQuantitative AssaysIn Vivo RelevanceElectroporation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2821?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/2821?article_type=v

In vivo Electroporation of Developing Mouse Retina
check_url/2821?article_type=v

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
check_url/2821?article_type=v

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
check_url/2821?article_type=v

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
check_url/2821?article_type=v

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
check_url/2821?article_type=v

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
check_url/2821?article_type=v

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
check_url/2821?article_type=v

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
check_url/2821?article_type=v

Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

Read Article