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高通量酵母质粒过表达屏幕
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JoVE Journal Biology
High-throughput Yeast Plasmid Overexpression Screen
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高通量酵母质粒过表达屏幕

DOI:
10.3791/2836-v

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08:57 min

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July 27, 2011

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,
1Neuroscience Graduate Group,University of Pennsylvania School of Medicine , 2Department of Cell and Developmental Biology,University of Pennsylvania School of Medicine

Chapters

  • 00:05Title
  • 00:56Preparations for Yeast Transformation
  • 02:19Yeast Transformation Using a Liquid Handling Robot
  • 06:04Spotting Assay
  • 07:10Representative Results: Plasmid Overexpression Screen
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一个质粒的过度表达屏幕<em>酿酒酵母</em>，使用液体处理机器人摆着质粒库和高通量酵母转化协议。

Tags

High-throughputYeastPlasmidOverexpression ScreenSaccharomyces CerevisiaeGenetic ScreensGene FunctionLiquid Handling RobotArrayed LibraryNeurodegenerative Disease ProteinsCellular Phenotypes

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