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流式细胞仪分析小鼠主动脉内流的免疫细胞
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
DOI:

15:15 min

July 01, 2011

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Chapters

  • 00:05Title
  • 01:39Isolation of Murine Aortas and Adventitia
  • 03:51Preparation of Aortic and Adventitial Single Cell Suspensions
  • 06:15Flow Cytometry Staining
  • 09:42Representative Flow Cytometric Analyses of Murine Aortic and Adventitial Infiltrating Leukocytes

Summary

Automatic Translation

本文介绍一个基于流式细胞仪的方法进行调查的主动脉免疫组成。该文件还说明了一个额外的技术,允许单独审视周围的外膜和血管壁。这种方法打开执行主动脉白细胞表型分析,适用于动脉粥样硬化研究的几种免疫检测的可能性。

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