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The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
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The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

DOI:
10.3791/2874-v

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07:19 min

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June 10, 2011

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1Department of Medical Pharmacology and Physiology,University of Missouri, 2Dalton Cardiovascular Research Center,University of Missouri

Chapters

  • 00:05Title
  • 01:16Preparations: The Microscope Stage and PSS Solutions
  • 02:53Preparation for Surgery
  • 03:41Surgical Preparations of the Open Cremaster Muscle
  • 06:16Intravital Imaging of the Cremaster Muscle Microcirculation
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and hamsters.

Tags

MouseCremaster Muscle PreparationIntravital ImagingMicrocirculationHomeostasisBlood SupplyArteriolesCapillariesVenulesOxygenated BloodDiffusional ExchangeExperimental ApproachVisualizingManipulatingInflammationHistologyElectron MicroscopyVasoactive DrugsReflected Light

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