Journal
/
Biology
/
超高分子量DNA提取微生物席
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Extraction of High Molecular Weight DNA from Microbial Mats
Please note that all translations are automatically generated. Click here for the English version.

超高分子量DNA提取微生物席

DOI:
10.3791/2887-v

•

09:30 min

•

July 07, 2011

•

, ,
1Department of Environmental Health Sciences,Arnold School of Public Health, University of South Carolina

Chapters

  • 00:05Title
  • 01:29Microbial Cell Extraction
  • 04:29DNA Extraction and Purification
  • 07:36Analysis of DNA Extracted from Microbial Mats
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

我们提供了一个从盐湖微生物席超高分子量DNA提取的改进协议。微生物细胞中分离DNA提取和纯化前的垫矩阵。这将增强的浓度，质量和大小的DNA。该协议可用于其他耐火材料样品。

Tags

High Molecular Weight DNAMicrobial MatsMetagenomic Data AnalysisDNA ExtractionHMW Community DNAEnvironmental Mat SamplesExtracellular Polymeric Substances (EPS)SaltsDirect LysisHarsh Extraction MethodsFragmented DNALarge-insert Vector CloningImproved MethodologyHypersaline Microbial MatsBlending And Differential CentrifugationMechanical And Chemical ProceduresDNA PurificationA260/280 Ratio16S RRNA Genes Amplification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2887?article_type=v

微生物群落的生物 - 面试
check_url/2887?article_type=v

从0.22微米Sterivex过滤器和氯化铯密度梯度离心法的DNA提取
check_url/2887?article_type=v

超高分子量基因组DNA的提取土壤和沉积物
check_url/2887?article_type=v

液相恢复GELFREE 8100分馏系统使用基于分子量分馏
check_url/2887?article_type=v

从石蜡包埋材料的遗传和表观遗传分析的DNA提取
check_url/2887?article_type=v

一种混合DNA提取方法细菌群落的定性和定量评估，从家禽生产样品
check_url/2887?article_type=v

粘蛋白琼脂糖凝胶电泳：蛋白质印迹高分子量糖蛋白
check_url/2887?article_type=v

福尔马林固定石蜡包埋的组织核心的制备RNA和DNA提取
check_url/2887?article_type=v

从白蛉提取内分泌物的方法<em> Bemisia tabaci</em
check_url/2887?article_type=v

改进的高通量DNA提取板加载方法，降低污染的可能性

Read Article