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A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract
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A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract

A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract

DOI:
10.3791/2912-v

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10:24 min

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August 19, 2011

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, , , , , , ,
1Department of Comparative Biosciences, School of Veterinary Medicine,University of Wisconsin-Madison

Chapters

  • 00:05Title
  • 01:42Preparing the Urogenital Tissue for Sectioning
  • 02:54Sectioning Urogenital Tissue with a Vibrating Microtome
  • 04:45Sample Basket Preparation
  • 05:34In situ Hybridization
  • 08:46Stained Lower Urogenital Tract Sections
  • 09:45Conclusion

Summary

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Automatic Translation

Here, we describe an efficient high throughput in situ hybridization (ISH) method for visualizing patterns of mRNA expression in developing fetal mouse prostate tissue sections. The method can be easily adapted to visualize mRNA expression patterns in other mouse tissues or in tissues from other species.

Tags

High ThroughputIn Situ HybridizationMRNA Expression PatternsFetal MouseLower Urogenital TractProstate DevelopmentAndrogenic SignalsEpithelial-mesenchymal InteractionsMolecular MechanismsBenign Prostatic HyperplasiaProstate CancerAnatomically ComplexProstatic BuddingCell TypesMesenchymal StromaAndrogen Receptor-dependent Paracrine SignalsStromal Androgen Receptor-responsive GenesProstate Ductal EpitheliumIn Situ Hybridization (ISH)Localization Of MRNAs

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