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An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth
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An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth

DOI:
10.3791/2914-v

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09:14 min

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August 11, 2011

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1Department of Biology,University of Haifa, 2Transgenic Oncogenesis and Genomics Section, Laboratory of Cancer Biology and Genetics,National Cancer Institute

Chapters

  • 00:05Title
  • 01:41Cell Proliferation Assay of Dormant (Quiescent) and Metastatic (Proliferating) Tumor Cells Cultured in a 3D-Basement Membrane Extract (BME) System
  • 04:14Immunofluorescent Staining for Cell Signaling Molecules in Dormant (Quiescent) Tumor Cells and/or Metastatic (Proliferating) Tumor Cells
  • 07:17Evaluation of Tumor Cell Proliferation and Dormancy Using the 3D-BME Culture System
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

A modified 3-D in vitro system is presented in which growth characteristics of several tumor cell lines in reconstituted basement membrane correlate with the dormant or proliferative behavior of the tumor cells at a metastatic secondary site in vivo.

Tags

In Vitro SystemTumor DormancyMetastatic GrowthBreast Cancer RecurrenceLatent PeriodMetastatic SitesConventional TherapiesDormant Cancer CellsQuiescent Solitary CellsDisease ProgressionBone MarrowBloodLymph NodesMechanisms Regulating DormancyProliferative StateModel SystemsMetastatic Progression

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