01:41Cell Proliferation Assay of Dormant (Quiescent) and Metastatic (Proliferating) Tumor Cells Cultured in a 3D-Basement Membrane Extract (BME) System
04:14Immunofluorescent Staining for Cell Signaling Molecules in Dormant (Quiescent) Tumor Cells and/or Metastatic (Proliferating) Tumor Cells
07:17Evaluation of Tumor Cell Proliferation and Dormancy Using the 3D-BME Culture System
A modified 3-D in vitro system is presented in which growth characteristics of several tumor cell lines in reconstituted basement membrane correlate with the dormant or proliferative behavior of the tumor cells at a metastatic secondary site in vivo.