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真菌内切葡聚糖酶的活性高通量筛选大肠埃希氏菌</em
JoVE Journal
Biology
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JoVE Journal Biology
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
DOI:

06:16 min

August 13, 2011

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Chapters

  • 00:05Title
  • 01:16Screening Plate Preparation
  • 02:07Endoglucanase Library Generation and Screen
  • 05:16Fungal Catalytic Domains in E. coli
  • 05:58Conclusion

Summary

Automatic Translation

我们描述了一种低成本,高通量方法真菌内切葡聚糖酶的活性在屏幕<em> E。大肠杆菌。</em>该方法依赖于一个简单的基质降解的视觉读数,不需要酶的纯化,并高度可伸缩的。这使得酶变异体的大型图书馆的快速筛选。

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