Journal
/
Neuroscience
/
Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

DOI:
10.3791/2951-v

•

11:34 min

•

May 22, 2011

•

, ,
1Department of Cell Biology and Human Anatomy, School of Medicine,University of California, Davis

Chapters

  • 00:05Title
  • 00:17Introduction
  • 01:24Preparation
  • 03:22Microinjection
  • 06:17Immunohistochemistry and Visualization
  • 09:36Results
  • 10:19Discussion and Conclusion

Summary

Automatic Translation

Automatic Translation

We present a lentiviral technique for genetic manipulation and visualization of single olfactory sensory neuron axon and its terminal arborization in vivo.

Tags

Lentivirus-mediatedGenetic ManipulationVisualizationOlfactory Sensory NeuronsIn VivoOlfactory CircuitProjectionAxonsSynaptic TargetsOlfactory BulbMolecular MechanismsGene ExpressionVisualizingSingle OSN AxonsTerminal Arbor MorphologyLentiviral Based TechniqueMicroinjectionOlfactory EpitheliumExpression CassettesGenome IntegrationGreen Fluorescent Protein ExpressionReporter DetectionGene Function StudiesDevelopment PeriodOver-expressionShRNA Mediated Knock-downMorphologiesCell Bodies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2951?article_type=v

Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording
check_url/2951?article_type=v

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
check_url/2951?article_type=v

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
check_url/2951?article_type=v

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
check_url/2951?article_type=v

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
check_url/2951?article_type=v

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
check_url/2951?article_type=v

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
check_url/2951?article_type=v

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
check_url/2951?article_type=v

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb
check_url/2951?article_type=v

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Read Article