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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Article DOI: 10.3791/3076
August 9th, 2011 •
1, 2, 2, 1
1St Vincent’s Institute, Department of Medicine, The University of Melbourne, 2Department of Microbiology and Immunology, The University of Melbourne

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August 9th, 2011

Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.

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