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JoVE Journal
Biology

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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
 

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Article DOI: 10.3791/3110
October 5th, 2011

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Summary October 5th, 2011

Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.

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