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JoVE Journal
Biology

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人类诱导多能干细胞的转染和Nucleofecting
 
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人类诱导多能干细胞的转染和Nucleofecting

Article DOI: 10.3791/3110-v 10:25 min October 5th, 2011
October 5th, 2011

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

尽管基因改造的最新进展,转染的人类胚胎干细胞(胚胎干细胞)保持一个反复无常的过程。据我们所知,系统和有效的方法,转染人诱导多能干细胞(iPSCs)尚未见报道。在这里,我们描述了可靠的协议,有效地转染和nucleofect人类iPSCs。

Tags

杂志,第56期,发育生物学,转染,iPS细胞,iPS细胞,胚胎干细胞,胚胎干细胞,核转染
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