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Transfektering och Nucleofecting Human inducerade pluripotenta stamceller
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Transfektering och Nucleofecting Human inducerade pluripotenta stamceller

Article DOI: 10.3791/3110-v • 10:25 min • October 5th, 2011
October 5th, 2011 •
1, 1, 1
1UCR Stem Cell Center, Department of Cell Biology and Neuroscience, University of California Riverside

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Trots den senaste tidens framsteg inom genetisk modifiering förblir transfektion av mänskliga embryonala stamceller (hESCs) en nyckfull process. Såvitt vi vet har systematiska och effektiva metoder för att transfektera humana inducerade pluripotenta stamceller (iPSCs) inte rapporterats. Här beskriver vi robusta protokoll för att effektivt transfektera och nucleofect mänskliga iPSCs.

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Medicin utvecklingsbiologi Transfektion iPS-celler IPSCs ES-celler hESCs Nucleofection
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