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Neural Tube Closure in Mouse Whole Embryo Culture
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neural Tube Closure in Mouse Whole Embryo Culture

Neural Tube Closure in Mouse Whole Embryo Culture

DOI:
10.3791/3132-v

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07:37 min

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October 21, 2011

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,
1Department of Neurology/Neuroscience,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:47Preparation of Culture Medium and Isolation of Uterus and Removal of Embryos
  • 03:59Setting up the Culture System
  • 05:20Evaluating Development after Whole Embryo Culture
  • 06:10Representative Embryo Cultures
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.

Tags

Neural Tube ClosureMouse EmbryosEmbryo CultureGenetic Mouse ModelsMammalian Neural Tube ClosureMaternal MetabolismPharmacological ManipulationSmall Molecule DeliveryRecombinant Protein DeliverySiRNA DeliveryWhole Embryo CultureEnriched MediaRoller Incubator ApparatusNormal Neural Tube ClosureIn Vitro TechniquesNeurulationNeural Plate FormationCranial Fold ClosureCaudal Neuropore ClosureEmbryo Dissection

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