JoVE Encyclopedia of Experiments
Neuroscience
0 views • 5:05 min • July 8th, 2025
Begin with an anesthetized mouse with an exposed skull. The mouse is secured in a stereotaxic frame containing a syringe pre-filled with aggregation-prone amyloid-beta proteins.
Mark the bregma, a key reference point for determining protein infusion coordinates.
Move the syringe, and touch the bregma, then the lambda, to determine the dorsal-ventral coordinates, ensuring precise needle depth.
Reposition the syringe above the predetermined medial-lateral coordinate, targeting the dentate gyrus, crucial for memory processing.
Touch the skull surface, ensuring the dorsal-ventral coordinate is within the limit, and mark it. Repeat this procedure on the opposite side.
Drill at the marked medial-lateral coordinate, inject the amyloid-beta proteins into the dentate gyrus, and hold to prevent backflow.
Drill and inject amyloid-beta proteins on the opposite side for uniform protein dispersion.
Detach the ear bars from the mouse, then suture the incision.
Over time, amyloid-beta proteins aggregate to form plaques that degrade dentate gyrus neurons and disrupt memory processing, resulting in Alzheimer's disease.
Make a 2- to 3-millimeter incision in the midline of the scalp with a scalpel. Us
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