JoVE Encyclopedia of Experiments
Neuroscience
0 views • 4:43 min • July 8th, 2025
Take an anesthetized mouse with a shaved neck.
Disinfect the neck and make a midline incision.
Expose the common carotid artery, or CCA, which bifurcates into the external carotid artery, or ECA, and internal carotid artery, or ICA.
Ligate the ECA and temporarily occlude both the CCA and ICA to restrict blood flow.
Incise the ECA, insert a filament, and advance it into the CCA.
Reopen the CCA and ICA to restore blood flow. Redirect the filament through the ICA toward the brain to perforate the anterior cerebral artery, a branch of the ICA.
The perforation causes bleeding, or hemorrhage, into the subarachnoid space, a fluid-filled area surrounding the brain. Confirm the hemorrhage by observing clinical symptoms.
Close the CCA to stop blood flow, remove the filament, and ligate the ECA incision.
Reopen the CCA to continue subarachnoid bleeding.
Suture the skin and allow the mouse to recover for analysis.
Begin by shaving the neck hair of the mouse. Apply ophthalmic ointment during the procedure. Sanitize the skin with 70% ethanol, followed by betadine or chlorhexidine, and apply 1% lidocaine. Place the mouse in a supine position with its head and torso facing an upward direction.
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