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Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

DOI:
10.3791/3145-v

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07:31 min

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July 28, 2011

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, , ,
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology,University of Groningen

Chapters

  • 00:05Title
  • 01:10Preparation of B. subtilis Cultures
  • 02:18Preparation of the Microscope Sample
  • 05:14Time-lapse Fluorescence Microscopy
  • 06:36Representative B. subtilis Outgrowth Data
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy. Furthermore, we provide guidelines how to analyze the microscopy images.

Tags

Live Cell ImagingBacillus SubtilisStreptococcus PneumoniaeAutomated Time-lapse MicroscopySingle Cell StudiesMicroscopy MethodFluorescence MicroscopyGrowth And DivisionPhylogenetic Lineage TreesCell History And AncestryGene Expression DynamicsProtein Localization

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